Two DITA2Go general configuration templates, d2htm_config.ini and d2rtf_config.ini, respectively reference local_d2htm_macros.ini and local_d2rtf_macros.ini; and these two editable macro library files in turn reference d2htm_macros.ini and d2rtf_macros.ini, respectively.
For example, in d2htm_config.ini:
[Templates]
; Macros = path to macro library
Macros = %OMSYSHOME%\d2g\local\macros\local_d2htm_macros.ini
Therefore you do not have to include a setting for Macros in your starting project configuration file, unless you wish to include an additional macro library in the chain. See §37.2.4 Including macro definitions in your own macro library.